FSTest Methomyl Rapid Test Kit (10 Tests)

Product Overview

The FSTest Methomyl Rapid Test Kit (Colloidal Gold) is a professional-grade, rapid screening tool engineered for the qualitative detection of methomyl residues in fresh fruits and vegetables. Methomyl is a highly toxic carbamate insecticide widely monitored in global agricultural trade. Ensuring strict residue tracking is vital for agricultural crop growers, food supply chain operators, and export compliance auditors striving to conform with global food safety standards and Maximum Residue Limits (MRLs).

By eliminating the immediate necessity for complex and costly laboratory infrastructure (such as HPLC or GC-MS), this field-ready assay compresses agricultural chemical extraction into a simplified, room-temperature workflow. It provides an essential first-line defense in fresh produce logistics, crop management, and corporate food safety compliance screening programs.

Product Highlights & Key Benefits

· High-Speed Field Turnaround: Complete the entire workflow and read stable, highly reliable visual results within just 5 to 8 minutes post-sample-preparation.

· Highly Sensitive Screening: Calibrated to yield dependable qualitative screenings at a strict Limit of Detection (LOD) of 0.2 mg/kg (ppm) across various fruit and vegetable matrices.

· Fully Self-Contained Kit Architecture: Arrives fully packed with all necessary consumables—including 10 test cassettes, 10 centrifuge tubes (2 mL), 1 bottle of dedicated extracting solution (70 mL), and 2 sampling bottles (30 mL).

· Definite Visual Interpretation: Utilizes a competitive lateral flow immunochromatographic assay format where the color intensity of the Test (T) line shares an opposite correspondence with the methomyl concentration, removing analytical ambiguity.

· High Specificity & Zero Cross-Reactivity: Proven to stay entirely unaffected by non-target agrochemicals. Samples containing 1 mg/L of common alternative treatments—including Chlorpyrifos, Carbendazim, Triazophos, and Chlorothalonil—consistently return negative results.

· Safe & Extended Shelf Life: Unopened test kits can be safely kept in a sealed, cool, and dry location between 4–30°C for an extended shelf-life of up to 18 months. Do not freeze.

Technical Principle: Lateral Flow Immunochromatography

The test strip is based on a competitive lateral flow immunochromatography mechanism. The test cassette houses an integrated observation window containing an invisible Test (T) zone and a Control (C) zone before fluidics activation.

Extracted Insecticide Residues + Antibody-Conjugates -> Lateral Flow -> Reaction with Pre-coated Recombinant Antigens -> Visual T & C Line Signal Interpretation

When a treated agricultural sample is dispensed into the sample well of the device, the liquid migrates laterally across the surface of the test strip via capillary action and reacts with pre-coated recombinant antigens. The intensity of color on the T test line has an opposite correspondence with the methomyl concentration in the sample. If the pesticide residue meets or exceeds the targeted detection threshold, it binds with the antibodies and alters the visual capture, causing the T line to fade visibly or disappear entirely. For a clean, negative sample, a strong, distinct red band forms at the T zone. The Control (C) line must always develop after a sample is applied to confirm the test fluidics are fully functional and valid.

Specifications & General Parameters

Step-by-Step Procedure

1. Simple Sample Extraction & Preparation

·  Comminution: Cut the fresh fruit or vegetable sample into small pieces (approx. 0.5 cm x 0.5 cm).

·  Primary Extraction: Weigh exactly 2 g of sample pieces into a sampling bottle and mix with 5 mL of the provided extracting solution.

·  Mixing & Settling: Vortex or shake the bottle thoroughly for 1 minute, then let it stand undisturbed for 1 minute. (Note: If the resulting supernatant liquid layer is cloudy or not completely clear, transfer 1 mL of fluid into a centrifuge tube and centrifuge at 4000 rpm for 3 minutes).

2. Running the Assay

· Prior to testing, ensure the test cassette has equilibrated to a proper operating temperature environment (20–40 degrees C) and place the device on a flat surface.

· Mount exactly 100 uL of the prepared final test solution directly into the sample well of the cassette.

· Interpret the visual results between 5 to 8 minutes. (Do not read after 8 minutes, as late chemical shifts render the test invalid).

Results Determination & Action Guide

· Negative (-): Both the Control (C) line and Test (T) line develop a strong, distinct red band. This confirms that methomyl insecticide residues are safely below the targeted threshold limit.

· Positive (+): The Control (C) line develops normally, but the Test (T) line is visibly lighter or fails to appear completely. This indicates that insecticide residues meet or exceed the designated threshold limit.

· Invalid: The Control (C) line fails to show any visible color band, regardless of the appearance of the T line. This signals an operational error, an uncalibrated pipette, or a compromised strip. The sample must be re-tested using a brand new cassette.

Storage & Packing Specifications

· Kit Capacity:10 tests per box (Test cassettes x 10, Extracting solution (70 mL) x 1, 30mL sampling bottles x 2, 2mL centrifuge tubes x 10, User manual).

· Storage Environment:Store tightly sealed in a cool, dry location between 4–30 degrees C. Do not freeze.

· Shelf Life:18 months under proper sealed storage parameters.