FSTest Cyromazine Rapid Test Kit (10 Tests)

Product Overview

The FSTest Cyromazine Rapid Test Kit (Colloidal Gold) is a professional-grade, rapid screening tool engineered for the qualitative detection of cyromazine residues in fresh fruits and vegetables. Cyromazine is a widely monitored triazine insect growth regulator used extensively in global crop protection and pest control. Ensuring strict residue tracking is vital for agricultural growers, fresh produce supply chain operators, and export compliance auditors striving to conform with international food safety standards and Maximum Residue Limits (MRLs).

By eliminating the immediate necessity for complex and costly laboratory infrastructure (such as HPLC or GC-MS), this field-ready assay compresses agricultural chemical extraction into a simplified, room-temperature workflow. It serves as an indispensable first-line defense in fresh produce logistics, harvesting hubs, and corporate food safety compliance screening programs.

Product Highlights & Key Benefits

· High-Speed Field Turnaround: Complete the entire screening assay and read stable, highly reliable visual results within just 8 to 10 minutes post-sample-preparation.

· Versatile Matrix Detection Limits: Calibrated to adapt dynamically across various agricultural matrices. By adjusting the sample supernatant dilution ratios, the kit dynamically targets regulatory cut-off thresholds ranging from 0.5 mg/kg up to 20 mg/kg.

· Extensive Fruit & Vegetable Adaptability: Officially validated across a diverse commodity matrix, including cowpea, common bean, edible-pod pea, pea, lentil, cantaloupe, mango, cucumber, broccoli, stem lettuce, bitter melon, zucchini, green onion, bell pepper, leaf lettuce, head lettuce, celery, ginger, leaf mustard, and sponge gourd.

· Definite Visual Interpretation: Utilizes a sandwich lateral flow immunochromatographic assay format where an increasing insecticide concentration causes the Test (T) line to fade or completely disappear, removing analytical ambiguity.

· Long-Term Ambient Preservation: Unopened test kits can be safely kept tightly sealed in a cool, dry location between 4–30°C for a full guaranteed shelf-life of up to 12 months, ensuring substantial logistical flexibility. Do not freeze.

· Safe & Non-Toxic Formulation: The specialized extraction solution supplied in the kit is certified as safe, non-toxic, and user-friendly for operators processing samples on-site.

Technical Principle: Lateral Flow Immunochromatography

The test strip is based on a sandwich lateral flow immunochromatography mechanism. The test cassette houses an integrated testing window containing an invisible Test (T) zone and a Control (C) zone before fluidics activation.

Extracted Insecticide Residues + Antibody-Conjugates -> Lateral Flow -> Reaction with Pre-coated Recombinant Antigens -> Faded or Absent T-Line (Inhibitory Response)

When a treated sample solution is dispensed into the sample well of the device, the liquid migrates laterally across the surface of the test strip via capillary action and reacts with pre-coated recombinant antigens. The intensity of color on the T test line has an opposite correspondence with the cyromazine concentration in the sample. If the pesticide residue meets or exceeds the targeted detection threshold, it alters the binding kinetics, causing the T line to fade visibly or disappear entirely. For a clean, negative sample, a strong, distinct red band forms at the T zone. The Control (C) line must always develop after a sample is applied to confirm the test fluidics are functional and valid.

Specifications & General Parameters

Step-by-Step Procedure

1. Simple Sample Extraction & Preparation

·  Comminution: Cut the fresh fruit or vegetable sample into small pieces (approx. 0.5 cm x 0.5 cm).

·  Primary Extraction: Weigh exactly 1 g of sample into a sampling bottle and mix with 5 mL of the provided extracting solution.

·  Mixing & Settling: Vortex or shake the bottle thoroughly for 1 minute, then let it stand undisturbed for 1 minute. (Note: If the resulting supernatant liquid layer is cloudy or not completely clear, transfer 1 mL of fluid into a centrifuge tube and centrifuge at 4000 rpm for 3 minutes).

·  Matrix Dilution: Execute the specific X & Y dilution protocol as defined in the matrix guide section above based on the commodity being verified.

2. Running the Assay

· Prior to testing, ensure the test cassette has equilibrated to a proper operating temperature environment (20–40 degrees C) and place the device on a flat surface.

· Mount exactly 100 uL of the prepared final test solution directly into the sample well of the cassette.

· Interpret the visual results between 8 to 10 minutes. (Do not read after 10 minutes, as late chemical shifts render the test invalid).

Results Determination & Action Guide

· Negative (-): Both the Control (C) line and Test (T) line develop a strong, distinct red band. This confirms that cyromazine residues are safely below the targeted regulatory threshold limit.

· Positive (+): The Control (C) line develops normally, but the Test (T) line is visibly lighter or fails to appear completely. This indicates that insecticide residues meet or exceed the designated threshold limit.

· Invalid: The Control (C) line fails to show any visible color band, regardless of the appearance of the T line. This signals an operational error, an uncalibrated pipette, or a compromised strip. The sample must be re-tested using a brand new cassette.

Storage & Packing Specifications

· Kit Capacity:10 tests per box (Test cassettes x 10, Centrifuge tubes (2mL) x 10, Extracting Solution (80mL) x 1, 30mL sampling bottles x 2, User manual).

· Storage Environment:Store tightly sealed in a cool, dry location between 4–30 degrees C. Do not freeze.

· Shelf Life:12 months under proper sealed storage parameters.