FSTest Histamine Rapid Test Strip
Product Overview
The FSTest Histamine Rapid Test Strip (Lateral Flow) is designed for the qualitative on-site screening of histamine residues in marine fish, freshwater fish, and their processed products. Based on a sandwich lateral flow immunochromatography assay, the test strip utilizes pre-coated recombinant antigens to deliver rapid, visual, and reliable results without requiring complex laboratory equipment.
Key Benefits
· Rapid Detection: Complete sample analysis with visual results in just 5 minutes.
· High Specificity: Optimized specifically for histamine detection across various aquatic product matrices.
· On-Site Convenience: Simple workflow with minimal equipment requirements, making it suitable for field testing, processing plants, and market regulation.
· Clear Signal Interpretation: Color intensity on the T-line inversely corresponds to the histamine concentration for unambiguous result reading.
Technical Specifications
Parameter | Specification |
Target Analyte | Histamine |
CAS Number | 51-45-6 |
Molecular Formula | C5H9N3 |
Molecular Weight | 111.15 |
Limit of Detection (LOD) | 30 mg/kg |
Pack Size | 25 tests/kit |
Assay Time | 5 minutes (results invalid after 7 minutes) |
Storage Conditions | Sealed at 4–30°C in a cool, dry place (Do not freeze) |
Shelf Life | 18 months |
Applicable Sample Types
· Marine fish
· Freshwater fish
· Fish products / Processed seafood products
Test Procedure
Step 1: Reagent Preparation — Dilute the 10× Histamine extracting solution with purified water at a 1:9 volume ratio before use.
Step 2: Sample Extraction — Mash 100g of representative sample. Weigh 1g into a 15mL centrifuge tube, add 10mL of diluted extracting solution, and shake vigorously for 3 minutes.
Step 3: Sample Dilution — Centrifuge at 4000 rpm for 1 minute. Mix 0.1mL of the supernatant with 0.9mL of histamine diluent and shake well for 30 seconds.
Step 4: Testing — Pipette 150µL of the prepared solution into a blank microwell. Insert the test strip into the microwell and read the result at 5 minutes.
Performance & Validation
The FSTest Histamine Rapid Test Strip is validated as a preliminary qualitative assay for fast screening of histamine contamination levels.
· Negative (-): Both C line and T line appear.
· Positive (+): C line appears; T line does not appear or is noticeably lighter than the control, indicating histamine presence at or above the LOD.
· Invalid: C line fails to appear, indicating a procedure or test strip failure.
