FSTest Diazinon Rapid Test Kit (10 Tests)

Product Overview

The FSTest Diazinon Rapid Test Kit (Colloidal Gold) is a professional-grade, rapid screening tool engineered for the qualitative detection of diazinon residues in agricultural products. Diazinon is a widely utilized organophosphorus insecticide and acaricide. Strict residue tracking is essential for agricultural producers, global food exporters, and laboratory auditors aiming to maintain strict compliance with global food safety regulations and Maximum Residue Limits (MRLs).

By bypassing the need for complex, time-consuming laboratory infrastructure (such as HPLC or GC-MS), this field-ready kit compresses agricultural sample extraction into a simplified, room-temperature workflow. It serves as an indispensable first-line defense in fresh produce supply chains, tea plantations, and corporate food safety compliance monitoring programs.

Product Highlights & Key Benefits

· High-Speed Field Turnaround: Complete the entire assay workflow and achieve highly reliable visual result readouts within just 5 to 8 minutes post-preparation.

· Matrix-Specific Limits of Detection: Uniquely calibrated to adapt dynamically across various agricultural matrices. By adjusting the sample supernatant dilution ratios, the kit targets a wide array of regulatory cut-off thresholds ranging from 50 ug/kg (0.05 mg/kg) up to 1 mg/kg.

· Fully Self-Contained Kit Architecture: Arrives fully packed with all necessary consumables—including 10 test cassettes, 10 microwells, 10 centrifuge tubes (2 mL), 1 bottle of Diazinon extracting solution (50 mL), and 2 sampling bottles (30 mL).

· Definite Visual Interpretation: Utilizes a competitive lateral flow immunochromatographic format where an increasing insecticide concentration causes the Test (T) line to fade or completely disappear, removing subjective reading errors.

· Ambient Transport & Stable Storage: Unopened test kits can be safely kept tightly sealed in a cool, dry location between 4–30°C for up to 12 months, providing substantial logistical flexibility. Do not freeze.

Technical Principle: Lateral Flow Immunochromatography

The test is based on a competitive lateral flow immunochromatographic assay format. The test cassette houses a pre-coated reaction membrane containing a Control (C) zone and an invisible Test (T) zone before fluidics activation.

Extracted Insecticide Residues + Antibody-Conjugates -> Lateral Flow -> Inhibition of Binding at T-Zone -> Faded or Absent T-Line

When the treated sample solution is dispensed into the sample hole on the device, the liquid migrates laterally across the strip via capillary action and reacts with pre-coated recombinant antigens. The color intensity of the T test line has an opposite correspondence with the diazinon concentration in the sample. If the pesticide residue meets or exceeds the target detection threshold, it binds to the antibodies and completely inhibits them from anchoring onto the antigens at the T zone, causing the T line to fade visibly or disappear entirely. For a clean, negative sample, a strong, distinct red band forms at the T zone. The Control (C) line must always develop to confirm the test fluidics are functional and valid.

Specifications & General Parameters

Step-by-Step Procedure

1. Simple Sample Extraction & Preparation

·  Comminution: Cut the fresh sample into small pieces (approx. 0.5 cm x 0.5 cm).

·  Primary Extraction: Weigh exactly 2 g of sample into a 30 mL sampling bottle and mix with 3 mL of extracting solution.

·  Mixing & Settling: Vortex or shake the bottle thoroughly for 1 minute, then let it stand undisturbed for 1 minute. (Note: If the resulting supernatant liquid layer is cloudy or not completely clear, transfer 1 mL of fluid and centrifuge at 4000 rpm for 3 minutes).

·  Final Matrix Dilution: Follow the exact matrix configuration detailed in the dilution guide table above based on the produce type being verified.

2. Running the Assay

· Prior to testing, ensure the test cassette has equilibrated to a proper operating temperature environment (20–40°C) and place the device on a flat surface.

· Pipette exactly 100 µL of the prepared final sample solution into a reagent microwell, mix gently 3 to 5 times with the pipette, and let it stand undisturbed for 3 minutes.

· Dispense exactly 100 µL of this mixed solution from the microwell directly into the round sample hole of the cassette.

· Interpret the visual results between 5 to 8 minutes. (Do not read after 8 minutes, as late chemical shifts render the test invalid).

Results Determination & Action Guide

· Negative (-): Both the Control (C) line and Test (T) line develop a strong, distinct red band. This confirms that diazinon residues are safely below the targeted regulatory cutoff threshold.

· Positive (+): The Control (C) line develops normally, but the Test (T) line is visibly lighter or fails to appear completely. This indicates that insecticide residues meet or exceed the designated threshold limit.

· Invalid: The Control (C) line fails to show any visible color band, regardless of the appearance of the T line. This signals an operational error, an uncalibrated pipette, or a compromised strip. The sample must be re-tested using a brand new cassette.

Storage & Packing Specifications

· Kit Capacity:10 tests per box (Test cassettes x 10, Microwells x 10, Centrifuge tubes (2 mL) x 10, Diazinon extracting solution (50 mL) x 1, 30 mL sampling bottles x 2, User manual).

· Storage Environment:Store tightly sealed in a cool, dry location between 4–30°C. Do not freeze.

· Shelf Life:12 months under proper sealed storage parameters.