FSTest Aflatoxin B1 Rapid Test Kit (10 Tests)

Product Overview

The FSTest Aflatoxin B1 Rapid Test Kit (Lateral Flow) is a professional-grade, rapid screening tool engineered for the qualitative detection of Aflatoxin B1 contamination in agricultural commodities. Aflatoxin B1 is one of the most potent naturally occurring mycotoxins and a heavily regulated carcinogen strictly monitored in global food logistics, feed milling, and grain trading safety protocols. Maintaining reliable, on-site toxin screening is vital for grain millers, edible oil processors, livestock feed manufacturers, and compliance auditors striving to conform with strict international food safety standards and strict regulatory contamination thresholds.

By eliminating the immediate necessity for complex and costly laboratory infrastructure (such as HPLC, LC-MS/MS, or specialized ELISA workbenches), this field-ready assay compresses hazardous mycotoxin extraction into a simplified, room-temperature workflow. It provides an indispensable first-line defense at grain collection hubs, seaport shipping containers, and factory intake inspection gates.

Product Highlights & Key Benefits

· High-Speed Field Turnaround: Complete the entire screening assay workflow and achieve highly reliable visual result readouts within just 5 to 8 minutes post-sample-preparation.

· Dynamic Matrix-Specific Detection Limits: Calibrated to adapt flexibly across diverse food and feed matrices. By adjusting the sample supernatant dilution ratios, the kit precisely targets international regulatory cutoff thresholds ranging from 5 µg/kg, 10 µg/kg, up to 20 µg/kg.

· Comprehensive Agricultural Matrix Adaptability: Intended and officially validated for cross-commodity verification, covering grains (corn, rice, peanuts, wheat, flour, oatmeal, barley, cereal flakes, and other hulled grains), fermented soy products, roasted nuts and seeds, vegetable oils (including peanut oil, corn oil, and others), and animal feed formulations.

· Definite Visual Interpretation: Utilizes a competitive lateral flow immunochromatographic assay format where the color development of the Test (T) line is inversely related to the toxin presence. If Aflatoxin B1 meets or exceeds the designated analytical threshold, the appearance of the T line is inhibited completely, removing reading ambiguity.

· High Specificity & Zero Cross-Reactivity: Engineered with highly specific recombinant antigen-antibody pairs. Samples containing up to 2 mg/kg of alternative major co-existing mycotoxins—including Zearalenone (ZEN), Deoxynivalenol (DON), Fumonisin (FB), Ochratoxin A (OTA), and T-2 Toxin—consistently test clean and negative.

· Fully Self-Contained Kit Architecture: Arrives fully packed with essential screening materials—including 10 test cassettes, 10 microwells, 1 bottle of extraction solution (60 mL), 1 bottle of diluent solution (10 mL), 10 centrifuge tubes (15 mL), and 10 centrifuge tubes (2 mL).

Technical Principle: Lateral Flow Immunochromatography

The test strip is based on a competitive inhibition lateral flow immunochromatography mechanism. The test cassette houses an integrated observation window containing an invisible Test (T) zone and a Control (C) zone before fluidics activation.

Extracted Mycotoxin Contamination + Reagent Antibodies -> Lateral Flow -> Competition with Pre-coated Recombinant Antigens -> Inhibited T-Line (Toxin Detected Response)

When a treated agricultural sample solution is applied into the sample well of the device, the liquid migrates laterally across the surface of the test strip via capillary action and reacts with pre-coated recombinant antigens. The intensity of color on the T test line has an opposite correspondence with the Aflatoxin B1 concentration in the sample. If the toxin level meets or exceeds the targeted limit of detection, it alters the binding kinetics, causing the T line to disappear entirely. For a clean, negative sample, a clear red band forms at the T zone. The Control (C) line must always develop after a sample is applied to confirm the test fluidics are functional and valid.

Specifications & General Parameters

Step-by-Step Procedure

1. Simple Sample Extraction & Preparation

·  Milling: Take 100 g of grain representative sample and grind it thoroughly. Weigh exactly 1 g of the ground sample and add it into a provided 15 mL centrifuge tube.

·  Primary Extraction: Add exactly 5 mL of the provided extraction solution into the tube, re-cap the tube tightly, and vortex for 2 minutes to mix.

·  Centrifugation: Separate the matrix layer by centrifuging the tube at 4000 rpm for 3 minutes.

·  Matrix Dilution: Pipette X µL of the clear supernatant layer into a 2 mL centrifuge tube, add Y µL of diluent solution according to the matrix guide table above, and vortex to mix for 10 seconds.

2. Running the Assay

· Prior to testing, ensure the test cassette has equilibrated to a proper operating temperature environment (20–40 degrees C) and place the device on a flat surface.

· Take the test cassette out of its bag. Pipette exactly 100 µL of the prepared final diluted solution into a reagent microwell, mix for 3 to 5 times using the pipette fluidics, and let it stand undisturbed for 3 minutes.

· Transfer all of the mixed solution from the microwell and mount it directly into the sample well of the test cassette.

· Interpret the visual results between 5 to 8 minutes. (Do not read after 8 minutes, as late chemical shifts render the test invalid).

Results Determination & Action Guide

· Negative (-): Both the Control (C) line and Test (T) line develop a strong, distinct red band. This confirms that Aflatoxin B1 residue levels are safely below the targeted regulatory cutoff threshold limit.

· Positive (+): The Control (C) line develops normally, but the Test (T) line fails to appear completely or is visibly inhibited. This indicates that mycotoxin contamination meets or exceeds the designated cutoff limit.

· Invalid: The Control (C) line fails to show any visible color band, regardless of the appearance of the T line. This signals an operational error, an uncalibrated pipette, or a compromised strip. The sample must be re-tested using a brand new cassette.

Storage & Packing Specifications

· Kit Capacity:10 tests per box (Test cassettes x 10, Microwells x 10, Extraction Solution (60mL) x 1, Diluent Solution (10mL) x 1, 15mL centrifuge tubes x 10, 2mL centrifuge tubes x 10, User manual).

· Storage Environment:Store tightly sealed in a cool, dry location between 4–30 degrees C. Do not freeze.

· Shelf Life:12 months under proper sealed storage parameters.