FSTest Thiamethoxam Rapid Test Kit (10 Tests)

Product Overview

The FSTest Thiamethoxam Rapid Test Kit (Colloidal Gold) is a professional-grade, rapid screening tool engineered for the qualitative detection of thiamethoxam residues in fresh vegetables and fruits. Thiamethoxam is a widely monitored second-generation neonicotinoid insecticide utilized extensively in global crop protection. Maintaining strict residue tracking is vital for agricultural growers, fresh food supply chain operators, and export compliance auditors striving to conform with international food safety standards and Maximum Residue Limits (MRLs).

By eliminating the immediate necessity for complex and costly laboratory infrastructure (such as HPLC or GC-MS), this field-ready assay compresses agricultural pesticide extraction into a simplified, room-temperature workflow. It provides an indispensable first-line defense in fresh produce logistics, crop harvesting points, and corporate food safety compliance screening programs.

Product Highlights & Key Benefits

· High-Speed Field Turnaround: Complete the entire screening assay and achieve highly reliable visual result readouts within just 5 to 8 minutes post-sample-preparation.

· Dynamic Multi-Tier Detection Limits: Calibrated to adapt flexibly across diverse food matrices. By utilizing a sophisticated multi-step supernatant dilution protocol, the kit precisely targets regulatory cut-off thresholds ranging from an ultra-sensitive 0.01 mg/kg up to 10 mg/kg.

· Extensive Commodity Matrix Matching: Officially validated for cross-commodity verification, covering legume vegetables (pod non-edible and edible beans), young corn spears, bananas, Chinese kale, okra, melons, cabbage, bitter melon, luffa, wax gourd, pumpkins, potatoes, onions, rhizome vegetables, apples, pears, solanaceous vegetables, flowering Chinese cabbage, tomatoes, peppers, chieh-qua, stem lettuce, celery, stone fruits, brassica vegetables, spinach, leeks, romaine lettuce, and leaf lettuce.

· Definite Visual Interpretation: Utilizes a competitive lateral flow immunochromatographic assay format where the color intensity of the Test (T) line shares an opposite correspondence with the thiamethoxam concentration, eliminating analytical ambiguity.

· Comprehensive Kit Architecture: Arrives fully equipped with essential screening consumables, including 10 test cassettes, 20 centrifuge tubes (2 mL), 2 sampling bottles (30 mL), and an extra-large 100 mL bottle of dedicated extracting solution.

· Reliable Ambient Preservation: Unopened test kits can be safely kept tightly sealed in a cool, dry location between 4–30°C for a full guaranteed shelf-life of up to 12 months. Do not freeze.

Technical Principle: Lateral Flow Immunochromatography

The test strip is based on a sandwich lateral flow immunochromatography mechanism. The test cassette houses an integrated testing window containing an invisible Test (T) zone and a Control (C) zone before fluidics activation.

Extracted Insecticide Residues + Reagents -> Lateral Flow -> Reaction with Pre-coated Recombinant Antigens -> Faded or Absent T-Line (Inhibitory Response)

When a treated sample solution is dispensed into the sample hole of the device, the liquid migrates laterally across the surface of the test strip via capillary action and reacts with pre-coated recombinant antigens. The intensity of color on the T test line has an opposite correspondence with the thiamethoxam concentration in the sample. If the pesticide residue meets or exceeds the targeted detection threshold, it alters the binding kinetics, causing the T line to fade visibly or disappear entirely. For a clean, negative sample, a strong, distinct red band forms at the T zone. The Control (C) line must always develop after a sample is applied to confirm the test fluidics are functional and valid.

Specifications & General Parameters

Step-by-Step Procedure

1. Simple Sample Extraction & Preparation

·  Comminution: Cut the fresh crop sample into small pieces (approx. 0.5 cm x 0.5 cm).

·  Primary Extraction: Weigh exactly 1 g of sample pieces into a 30 mL sampling bottle and mix with 6 mL of the provided extracting solution.

·  Mixing & Settling: Vortex or shake the bottle thoroughly for 1 minute, then let it stand undisturbed for 1 minute to allow layers to separate. (Note: If the resulting supernatant liquid layer is cloudy or turbid, transfer 1 mL of fluid into a centrifuge tube and centrifuge at 4000 rpm for 3 minutes to obtain clear test solution).

·  Matrix Dilution: Execute the exact multi-tier matrix dilution protocol (Step 1 and Step 2) using the provided 2 mL centrifuge tubes as defined in the guide section above based on the specific commodity being verified.

2. Running the Assay

· Prior to testing, ensure the test cassette has equilibrated to a proper operating temperature environment (20–40 degrees C) and place the device on a flat surface.

· Mount exactly 100 µL of the final prepared sample solution directly into the sample hole of the cassette.

· Interpret the visual results between 5 to 8 minutes. (Do not read after 8 minutes, as late chemical shifts render the test invalid).

Results Determination & Action Guide

· Negative (-): Both the Control (C) line and Test (T) line develop color normally. This confirms that thiamethoxam insecticide residues are safely below the targeted regulatory cutoff threshold limit.

· Positive (+): The Control (C) line develops normally, but the Test (T) line is visibly lighter or fails to appear completely. This indicates that insecticide residues meet or exceed the designated threshold limit.

· Invalid: The Control (C) line fails to show any visible color band, regardless of the appearance of the T line. This signals an operational error, an uncalibrated pipette, or a compromised strip. The sample must be re-tested using a brand new cassette.

Storage & Packing Specifications

· Kit Capacity:10 tests per box (Test cassettes x 10, Centrifuge tubes (2 mL) x 20, Extracting solution (100 mL) x 1, 30 mL sampling bottles x 2, User manual).

· Storage Environment:Store tightly sealed in a cool, dry location between 4–30 degrees C. Do not freeze.

· Shelf Life:12 months under proper sealed storage parameters.