FSTest Quinolones Rapid Test Kit (Colloidal Gold)

Product Overview

The FSTest Quinolones Rapid Test Kit (Colloidal Gold) is an immunochromatographic assay designed for the qualitative detection of quinolone antibiotic residues in animal-derived foods. Based on a sandwich lateral flow immunochromatography principle, the test cassette utilizes pre-coated recombinant antigens to deliver rapid, visual, and reliable results directly observed by the naked eye without requiring complex laboratory equipment.

Key Benefits

· Broad Multi-Residue Screening: Detects 14 major quinolone drugs simultaneously in a single assay.

· Flexible Pretreatment Options: Features Method 1 for ultra-low LOD detection and Method 2 for rapid, streamlined field testing.

· Wide Sample Compatibility: Optimized for livestock, poultry, seafood tissues, and whole egg matrices.

· Rapid & Visual Interpretation: Clear visual result reading in just 8–10 minutes.

Technical Specifications

Applicable Sample Types

· Livestock & Poultry Tissue: Pork, beef, mutton, chicken, etc. (skin and fat removed)

· Seafood Tissue: Fish, shrimp, and other aquatic products

· Egg Products: Shelled and homogenized egg samples

Test Procedure

Step 1: Sample Preparation — Method 1 (High Sensitivity / Concentrated)
Weigh 3.0 ± 0.1g minced sample (skin/fat removed) or shelled egg into a 15mL centrifuge tube. Add 3mL Extracting Solution A, shake vigorously for 3 min. Add 1 bag Extracting Solution B, shake for 2 min. Centrifuge at 4000 rpm for 5 min. Transfer all supernatant to a 10mL tube, evaporate to dryness with hot air at 65°C. Add 0.5mL Purifying Agent and 0.3mL Diluent Solution, vortex for 1 min, and let stand to separate. The lower layer liquid is ready for testing.

Step 2: Sample Preparation — Method 2 (Rapid Field Screening)
Weigh 1.0 ± 0.1g minced sample or shelled egg into a 15mL centrifuge tube. Add 10mL Extracting Solution C, mix up and down for 2 min, centrifuge at 4000 rpm for 3 min. Pipette 0.1mL upper supernatant into a 10mL tube, add 2mL Diluent Solution, and vortex for 1 min. The liquid is ready for testing.

Step 3: Assay Execution & Result Reading
Ensure cassette is at 20–40°C. Pipette 100µL of prepared sample solution into a microwell, mix 3–5 times, and let stand for 3 min. Transfer all mixed solution to the sample well of the test cassette. Read and interpret results after 8–10 minutes (invalid after 10 minutes).

Performance & Validation

Limits of Detection (LODs) for Target Quinolone Residues (µg/kg):

Result Interpretation:

· Negative (-): Both C (Control) line and T (Test) line appear.

· Positive (+): C line appears; T line does not appear (color intensity on T line is inversely proportional to quinolones concentration).

· Invalid: C line fails to appear, regardless of T line presence.