FSTest Cyhalothrin Rapid Test Kit (10 Tests)

Product Overview

The FSTest Cyhalothrin Rapid Test Kit (Colloidal Gold) is a professional-grade, rapid screening tool engineered for the qualitative detection of cyhalothrin residues in fresh fruits and vegetables. Cyhalothrin is a widely monitored pyrethroid insecticide utilized extensively in global crop protection. Ensuring strict residue tracking is vital for agricultural growers, food supply chain operators, and export compliance auditors striving to conform with international food safety standards and Maximum Residue Limits (MRLs).

By eliminating the immediate necessity for complex and costly laboratory infrastructure (such as HPLC or GC-MS), this field-ready assay compresses agricultural pesticide extraction into a simplified, room-temperature workflow. It provides an indispensable first-line defense in fresh produce logistics, crop harvesting points, and corporate food safety compliance screening programs.

Product Highlights & Key Benefits

· High-Speed Field Turnaround: Complete the entire screening assay and achieve highly reliable visual result readouts within just 5 to 8 minutes post-sample-preparation.

· Versatile Matrix Detection Limits: Calibrated to adapt dynamically across various agricultural matrices. By adjusting the sample supernatant dilution ratios, the kit targets a wide array of regulatory cut-off thresholds ranging from 0.2 mg/kg up to 5 mg/kg.

· Extensive Fruit & Vegetable Adaptability: Officially validated across a diverse commodity matrix, including bulb vegetables, eggplants, heading cruciferous vegetables, broccoli, leafy greens, stone fruits (peach/nectarine), pome fruits (apple/pear), and tropical fruits (lychee).

· Definite Visual Interpretation: Utilizes a competitive sandwich lateral flow immunochromatographic assay format where an increasing insecticide concentration causes the Test (T) line to fade, completely removing subjective reading errors.

· High Specificity & Zero Cross-Reactivity: Proven to stay entirely unaffected by non-target agrochemicals. Samples containing 10 mg/kg of alternative common treatments—including Thiamethoxam, Triazophos, Chlorothalonil, Carbofuran, and Omethoate—consistently return clear negative results.

· Safe & Non-Toxic Formulation: The specialized extraction solution supplied in the kit is certified as safe, non-toxic, and user-friendly for operators processing samples on-site.

Technical Principle: Lateral Flow Immunochromatography

The test strip is based on a sandwich lateral flow immunochromatography mechanism. The test cassette houses an integrated testing window containing an invisible Test (T) zone and a Control (C) zone before fluidics activation.

Extracted Insecticide Residues + Antibody-Conjugates -> Lateral Flow -> Reaction with Pre-coated Recombinant Antigens -> Faded or Absent T-Line (Inhibitory Response)

When a treated sample solution is dispensed into the sample well of the device, the liquid migrates laterally across the surface of the test strip via capillary action and reacts with pre-coated recombinant antigens. The intensity of color on the T test line has an opposite correspondence with the cyhalothrin concentration in the sample. If the pesticide residue meets or exceeds the targeted detection threshold, it alters the binding kinetics, causing the T line to fade visibly or disappear entirely. For a clean, negative sample, a strong, distinct red band forms at the T zone. The Control (C) line must always develop after a sample is applied to confirm the test fluidics are functional and valid.

Specifications & General Parameters

Step-by-Step Procedure

1. Simple Sample Extraction & Preparation

·  Comminution: Cut the fresh fruit or vegetable sample into small pieces (approx. 0.5 cm x 0.5 cm).

·  Primary Extraction: Weigh exactly 2 g of sample into a sampling bottle and mix with 3 mL of the provided extracting solution.

·  Mixing & Settling: Vortex or shake the bottle thoroughly for 1 minute, then let it stand undisturbed for 1 minute. (Note: If the resulting supernatant liquid layer is cloudy or not completely clear, transfer 1 mL of fluid into a centrifuge tube and centrifuge at 4000 rpm for 3 minutes).

·  Matrix Dilution: Execute the specific X & Y dilution protocol as defined in the matrix guide section above based on the commodity being verified.

2. Running the Assay

· Prior to testing, ensure the test cassette has equilibrated to a proper operating temperature environment (20–40 degrees C) and place the device on a flat surface.

· Pipette exactly 120 µL of the prepared final test solution into a reagent microwell, mix for 3 to 5 times using the pipette, and let it stand undisturbed for 3 minutes.

· Transfer all of the mixed solution from the microwell directly into the round sample well on the test cassette.

· Interpret the visual results between 5 to 8 minutes. (Do not read after 8 minutes, as late chemical shifts render the test invalid).

Results Determination & Action Guide

· Negative (-): Both the Control (C) line and Test (T) line develop a strong, distinct red band. This confirms that cyhalothrin residues are safely below the targeted regulatory threshold limit.

· Positive (+): The Control (C) line develops normally, but the Test (T) line is visibly lighter or fails to appear completely. This indicates that insecticide residues meet or exceed the designated threshold limit.

· Invalid: The Control (C) line fails to show any visible color band, regardless of the appearance of the T line. This signals an operational error, an uncalibrated pipette, or a compromised strip. The sample must be re-tested using a brand new cassette.

Storage & Packing Specifications

· Kit Capacity:10 tests per box (Test cassettes x 10, Microwells x 10, Centrifuge tubes (2mL) x 10, Extracting Solution (50mL) x 1, 30mL sampling bottles x 2, User manual).

· Storage Environment:Store tightly sealed in a cool, dry location between 4–30 degrees C. Do not freeze.

· Shelf Life:12 months under proper sealed storage parameters.