FSTest Profenofos Rapid Test Kit (10 Tests)

Product Overview

The FSTest Profenofos Rapid Test Kit (Colloidal Gold) is a professional-grade, rapid screening tool engineered for the qualitative detection of profenofos residues in fresh fruits and vegetables. Profenofos is a widely applied organophosphorus insecticide and acaricide. Ensuring strict residue tracking is vital for agricultural crop growers, food supply chain operators, and export compliance auditors striving to conform with global food safety standards and Maximum Residue Limits (MRLs).

By eliminating the immediate necessity for complex and costly laboratory infrastructure (such as HPLC or GC-MS), this field-ready assay compresses agricultural chemical extraction into a simplified, room-temperature workflow. It provides an essential first-line defense in fresh produce logistics, crop management, and corporate food safety compliance screening programs.

Product Highlights & Key Benefits

· High-Speed Field Turnaround: Complete the entire workflow and read stable, highly reliable visual results within just 5 to 8 minutes post-sample-preparation.

· Versatile Matrix Detection Limits: Calibrated to yield dependable qualitative screenings across various food matrices. By adjusting the sample supernatant dilution ratios, the kit dynamically targets regulatory cut-off thresholds ranging from 0.5 mg/kg up to 10 mg/kg.

· Targeted Application Matrix: Validated for specific adaptability across highly relevant commodities, covering Head cabbage, Radish, Cauliflower, Chinese kale (Kai-lan), Chili pepper, Bok choy, Radish leaves, Tomato, and Mangosteen.

· Definite Visual Interpretation: Utilizes a competitive inhibition immunochromatography assay format where the color intensity of the Test (T) line shares an opposite correspondence with the profenofos concentration, removing analytical ambiguity.

· High Specificity & Zero Cross-Reactivity: Proven to stay entirely unaffected by non-target agrochemicals. Samples containing up to 10 mg/L of common alternative treatments—including Chlorpyrifos, Acetamiprid, Triazophos, and Chlorothalonil—consistently return negative results.

· Safe & Non-Toxic Formulation: The specialized extraction solution supplied in the kit is certified as safe and non-toxic for operators, ensuring hassle-free processing on-site.

Technical Principle: Lateral Flow Immunochromatography

The test strip is based on a competitive inhibition immunochromatography mechanism. The test cassette houses an integrated observation window containing an invisible Test (T) zone and a Control (C) zone before fluidics activation.

Extracted Insecticide Residues + Antibody-Conjugates -> Lateral Flow -> Reaction with Pre-coated Recombinant Antigens -> Visual T & C Line Signal Interpretation

When a treated agricultural sample is dispensed into the sample well of the device, the liquid migrates laterally across the surface of the test strip via capillary action and reacts with pre-coated recombinant antigens. The intensity of color on the T test line has an opposite correspondence with the profenofos concentration in the sample. If the pesticide residue meets or exceeds the targeted detection threshold, it binds with the antibodies and alters the visual capture, causing the T line to fade visibly or disappear entirely. For a clean, negative sample, a strong, distinct red band forms at the T zone. The Control (C) line must always develop after a sample is applied to confirm the test fluidics are fully functional and valid.

Specifications & General Parameters

Step-by-Step Procedure

1. Simple Sample Extraction & Preparation

·  Comminution: Cut the fresh fruit or vegetable sample into small pieces (approx. 0.5 cm x 0.5 cm).

·  Primary Extraction: Weigh exactly 2 g of representative sample into a sampling bottle and mix with 3 mL of extracting solution.

·  Mixing & Settling: Vortex or shake the bottle thoroughly for 1 minute, then let it stand undisturbed for 1 minute. (Note: If the resulting supernatant liquid layer is cloudy or not completely clear, transfer 1 mL of fluid into a centrifuge tube and centrifuge at 4000 rpm for 3 minutes).

·  Matrix Dilution: Execute the specific X & Y dilution protocol as defined in the matrix guide section above based on the commodity being verified.

2. Running the Assay

· Prior to testing, ensure the test cassette and solution have equilibrated to a proper room temperature environment (20–40 degrees C) and place the device on a flat surface.

· Mount exactly 100 uL of the prepared final sample solution directly into the sample hole of the cassette.

· Interpret the visual results between 5 to 8 minutes. (Do not read after 8 minutes, as late chemical shifts render the test invalid).

Results Determination & Action Guide

· Negative (-): Both the Control (C) line and Test (T) line develop a strong, distinct red band. This confirms that profenofos insecticide residues are safely below the targeted regulatory threshold limit.

· Positive (+): The Control (C) line develops normally, but the Test (T) line is visibly lighter or fails to appear completely. This indicates that insecticide residues meet or exceed the designated threshold limit.

· Invalid: The Control (C) line fails to show any visible color band, regardless of the appearance of the T line. This signals an operational error, an uncalibrated pipette, or a compromised strip. The sample must be re-tested using a brand new cassette.

Storage & Packing Specifications

· Kit Capacity:10 tests per box (Test cassettes x 10, Centrifuge tubes (2mL) x 10, Extracting Solution (50mL) x 1, 30mL sampling bottles x 2, User manual).

· Storage Environment:Store tightly sealed in a cool, dry location between 4–30 degrees C. Do not freeze.

· Shelf Life:12 months under proper sealed storage parameters.