FSTest Aldicarb Rapid Test Kit (10 Tests)

Product Overview

The FSTest Aldicarb Rapid Test Kit (Colloidal Gold) is a professional-grade, rapid screening tool engineered for the qualitative detection of aldicarb residues in fresh fruits and vegetables. Aldicarb is an extremely potent carbamate insecticide and nematicide subject to rigorous international agricultural enforcement. Maintaining strict residue tracking is vital for agricultural growers, fresh food exporters, and laboratory auditors striving to comply with stringent global food safety regulations and Maximum Residue Limits (MRLs).

By eliminating the immediate necessity for complex and costly laboratory infrastructure (such as HPLC or GC-MS), this field-ready assay compresses pesticide residue extraction into a simplified, room-temperature workflow. It serves as an indispensable first-line defense in fresh produce logistics, crop harvesting hubs, and corporate quality compliance screening programs.

Product Highlights & Key Benefits

· High-Speed Field Turnaround: Complete the entire screening assay workflow and read stable, highly reliable visual results within just 8 to 10 minutes post-sample-preparation.

· Ultra-High Sensitivity Range: Specifically calibrated to yield dependable qualitative screenings for major produce matrices at extremely low thresholds, targeting 0.02 mg/kg for fruits and down to a baseline limit of detection (LOD) of 20 µg/kg (0.02 mg/kg).

· Multi-Matrix Crop Adaptability: Officially validated across a massive agricultural commodity selection, including kernel/stone fruits, citrus, berries, melons, bulb/brassica vegetables, leafy greens, solanaceous crops, stems, aquatic vegetables, and root/tuber crops (including potatoes, sweet potatoes, and yams).

· Definite Visual Interpretation: Utilizes a sandwich lateral flow immunochromatographic format where the color intensity of the Test (T) line shares an opposite correspondence with the aldicarb concentration, removing subjective reading errors.

· Fully Self-Contained Consumable Kit: Arrives fully packed with all essential screening supplies—including 10 test cassettes, 10 microwells, 10 centrifuge tubes (2 mL), 1 bottle of dedicated extracting solution (50 mL), and 2 sampling bottles (30 mL).

· Reliable Ambient Preservation: Unopened test kits can be safely kept tightly sealed in a cool, dry location between 4–30°C for a full guaranteed shelf-life of up to 12 months. Do not freeze.

Technical Principle: Lateral Flow Immunochromatography

The test strip is based on a sandwich lateral flow immunochromatography assay mechanism. The test cassette houses an integrated testing window containing an invisible Test (T) zone and a Control (C) zone before fluidics activation.

Extracted Insecticide Residues + Antibody-Conjugates -> Lateral Flow -> Reaction with Pre-coated Recombinant Antigens -> Faded or Absent T-Line (Inhibitory Response)

When a treated sample solution is dispensed into the sample hole on the device, the liquid migrates laterally across the surface of the test strip via capillary action and reacts with pre-coated recombinant antigens. The intensity of color on the T test line has an opposite correspondence with the aldicarb concentration in the sample. If the pesticide residue meets or exceeds the targeted detection threshold, it alters the binding kinetics, causing the T line to fade visibly or disappear entirely. For a clean, negative sample, a strong, distinct red band forms at the T zone. The Control (C) line must always develop after a sample is applied to confirm the test fluidics are functional and valid.

Specifications & General Parameters

Step-by-Step Procedure

1. Simple Sample Extraction & Preparation

·  Comminution: Cut the fresh fruit or vegetable sample into small pieces (approx. 0.5 cm x 0.5 cm).

·  Primary Extraction: Weigh exactly 2 g of sample into a sampling bottle and mix with 5 mL of the provided extracting solution.

·  Mixing & Settling: Vortex or shake the bottle thoroughly for 1 minute, then let it stand undisturbed for 1 minute to allow layers to separate. (Note: If the resulting supernatant liquid layer is cloudy or turbid, transfer 1 mL of fluid into a centrifuge tube and centrifuge at 4000 rpm for 3 minutes. The obtained supernatant is used for subsequent dilution).

·  Matrix Dilution: Execute the specific X & Y matrix-matching dilution protocol as defined in the guide section above using the provided 2 mL centrifuge tubes.

2. Running the Assay

· Prior to testing, ensure the test cassette has equilibrated to a proper operating temperature environment (20–40 degrees C) and place the device on a flat surface.

· Pipette exactly 100 uL of the prepared final sample solution into a reagent microwell, mix for 3 to 5 times using the pipette fluidics, and let it stand undisturbed for 3 minutes.

· Transfer 100 uL of this mixed solution from the microwell directly into the sample hole on the test cassette.

· Interpret the visual results between 8 to 10 minutes. (Do not read after 10 minutes, as late chemical shifts render the test invalid).

Results Determination & Action Guide

· Negative (-): Both the Control (C) line and Test (T) line develop color normally. This confirms that aldicarb residues are safely below the targeted regulatory cutoff threshold.

· Positive (+): The Control (C) line develops normally, but the Test (T) line is visibly lighter or fails to appear completely. This indicates that insecticide residues meet or exceed the designated threshold limit.

· Invalid: The Control (C) line fails to show any visible color band, regardless of the appearance of the T line. This signals an operational error, an uncalibrated pipette, or a compromised strip. The sample must be re-tested using a brand new cassette.

Storage & Packing Specifications

· Kit Capacity:10 tests per box (Test cassettes x 10, Microwells x 10, Centrifuge tubes (2 mL) x 10, Extracting solution (50 mL) x 1, 30 mL sampling bottles x 2, User manual).

· Storage Environment:Store tightly sealed in a cool, dry location between 4–30 degrees C. Do not freeze.

· Shelf Life:12 months under proper sealed storage parameters.